ssr_primer
Primer Details for TRINITY_DN66498_c1_g1_i3 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCATCTAGGTGTTTGGGT | GGTGAATTATTGACGGCGGC | Start: 1671 |
| Tm | 60.035 | 59.971 | End: 1859 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCGTCCTTCCTCCTTTCACA | GTATGTCGCCACTGGTGTCA | Start: 1357 |
| Tm | 59.965 | 60.038 | End: 1564 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | CCCAAACACCTAGATGGCGT | Start: 1545 |
| Tm | 60.038 | 60.035 | End: 1689 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | ACCCAAACACCTAGATGGCG | Start: 1545 |
| Tm | 60.038 | 60.035 | End: 1690 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | TTCGTTCCGATGATGCACCA | Start: 1545 |
| Tm | 60.038 | 60.037 | End: 1709 |
| Size | 20 | 20 | 165 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCATCTAGGTGTTTGGGT | GGTGAATTATTGACGGCGGC | Start: 1671 |
| Tm | 60.035 | 59.971 | End: 1859 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCGTCCTTCCTCCTTTCACA | GTATGTCGCCACTGGTGTCA | Start: 1357 |
| Tm | 59.965 | 60.038 | End: 1564 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | CCCAAACACCTAGATGGCGT | Start: 1545 |
| Tm | 60.038 | 60.035 | End: 1689 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | ACCCAAACACCTAGATGGCG | Start: 1545 |
| Tm | 60.038 | 60.035 | End: 1690 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TGACACCAGTGGCGACATAC | TTCGTTCCGATGATGCACCA | Start: 1545 |
| Tm | 60.038 | 60.037 | End: 1709 |
| Size | 20 | 20 | 165 |
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