ssr_primer
Primer Details for TRINITY_DN66494_c0_g2_i1 |
|---|
| (T)29: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCATGTATGCCCCATGTT | AACGACAGACGCGCTTAAGA | Start: 1682 |
| Tm | 60.032 | 60.04 | End: 1946 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | TCCATGGGTTGTCGCGTTTA | GCTTACTTTCCCGCGTTCAC | Start: 306 |
| Tm | 59.965 | 59.835 | End: 430 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | TGCCATGTATGCCCCATGTT | ACGACAGACGCGCTTAAGAT | Start: 1682 |
| Tm | 60.032 | 59.829 | End: 1945 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AATGTAGGAGCGTGCCTCAG | TGGCGAGATCCTCTTCCTCA | Start: 1243 |
| Tm | 59.823 | 60.033 | End: 1381 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | AATCTCCTCCGTGGCTAGGT | GACGCGCTTAAGATGGTCCT | Start: 1663 |
| Tm | 60.032 | 60.179 | End: 1939 |
| Size | 20 | 20 | 277 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCATGTATGCCCCATGTT | AACGACAGACGCGCTTAAGA | Start: 1682 |
| Tm | 60.032 | 60.04 | End: 1946 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | TCCATGGGTTGTCGCGTTTA | GCTTACTTTCCCGCGTTCAC | Start: 306 |
| Tm | 59.965 | 59.835 | End: 430 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | TGCCATGTATGCCCCATGTT | ACGACAGACGCGCTTAAGAT | Start: 1682 |
| Tm | 60.032 | 59.829 | End: 1945 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AATGTAGGAGCGTGCCTCAG | TGGCGAGATCCTCTTCCTCA | Start: 1243 |
| Tm | 59.823 | 60.033 | End: 1381 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | AATCTCCTCCGTGGCTAGGT | GACGCGCTTAAGATGGTCCT | Start: 1663 |
| Tm | 60.032 | 60.179 | End: 1939 |
| Size | 20 | 20 | 277 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCATGTATGCCCCATGTT | AACGACAGACGCGCTTAAGA | Start: 1682 |
| Tm | 60.032 | 60.04 | End: 1946 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | TCCATGGGTTGTCGCGTTTA | GCTTACTTTCCCGCGTTCAC | Start: 306 |
| Tm | 59.965 | 59.835 | End: 430 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | TGCCATGTATGCCCCATGTT | ACGACAGACGCGCTTAAGAT | Start: 1682 |
| Tm | 60.032 | 59.829 | End: 1945 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AATGTAGGAGCGTGCCTCAG | TGGCGAGATCCTCTTCCTCA | Start: 1243 |
| Tm | 59.823 | 60.033 | End: 1381 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | AATCTCCTCCGTGGCTAGGT | GACGCGCTTAAGATGGTCCT | Start: 1663 |
| Tm | 60.032 | 60.179 | End: 1939 |
| Size | 20 | 20 | 277 |
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