ssr_primer
Primer Details for TRINITY_DN66491_c6_g3_i2 |
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| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGAAGCCTCCGTGACATT | TCGCTCTTTGTCTTCGTCCG | Start: 250 |
| Tm | 60.036 | 60.387 | End: 350 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | AAGGAGCCATAGAAACCGCG | AATGTCACGGAGGCTTCCAG | Start: 130 |
| Tm | 60.462 | 60.036 | End: 269 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
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| Sequence | AGAAACCGCGGCTATCTTCG | AATGTCACGGAGGCTTCCAG | Start: 140 |
| Tm | 60.528 | 60.036 | End: 269 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | CGGCTATCTTCGTCCGAACC | AATGTCACGGAGGCTTCCAG | Start: 148 |
| Tm | 60.594 | 60.036 | End: 269 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
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| Sequence | GCCATAGAAACCGCGGCTAT | AATGTCACGGAGGCTTCCAG | Start: 135 |
| Tm | 60.603 | 60.036 | End: 269 |
| Size | 20 | 20 | 135 |
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