ssr_primer
Primer Details for TRINITY_DN66474_c0_g1_i5 |
|---|
| (CGT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTGTGCTATACTCGAGCG | CTTGCACGCTCCCGAGATAT | Start: 135 |
| Tm | 59.971 | 59.967 | End: 297 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGCTGTGCTATACTCGAGCG | GAAGTCGGGGCTATCCATGG | Start: 135 |
| Tm | 59.971 | 59.965 | End: 234 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ATATCTCGGGAGCGTGCAAG | GGATGACAGTGTCCCACGTT | Start: 278 |
| Tm | 59.967 | 59.965 | End: 419 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | AAGGAAATACACGGTGCGGT | CTTGCACGCTCCCGAGATAT | Start: 171 |
| Tm | 59.964 | 59.967 | End: 297 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TCCTCTACGATGACACCGGT | AAGTCATTGGCCTCTGCTCC | Start: 347 |
| Tm | 60.035 | 60.035 | End: 596 |
| Size | 20 | 20 | 250 |
(A)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTGTGCTATACTCGAGCG | CTTGCACGCTCCCGAGATAT | Start: 135 |
| Tm | 59.971 | 59.967 | End: 297 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGCTGTGCTATACTCGAGCG | GAAGTCGGGGCTATCCATGG | Start: 135 |
| Tm | 59.971 | 59.965 | End: 234 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ATATCTCGGGAGCGTGCAAG | GGATGACAGTGTCCCACGTT | Start: 278 |
| Tm | 59.967 | 59.965 | End: 419 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | AAGGAAATACACGGTGCGGT | CTTGCACGCTCCCGAGATAT | Start: 171 |
| Tm | 59.964 | 59.967 | End: 297 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TCCTCTACGATGACACCGGT | AAGTCATTGGCCTCTGCTCC | Start: 347 |
| Tm | 60.035 | 60.035 | End: 596 |
| Size | 20 | 20 | 250 |
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