ssr_primer
Primer Details for TRINITY_DN66473_c5_g1_i3 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTACACACGCGGTTCCAAC | TCCGCATTTATATCACTCGGGG | Start: 143 |
| Tm | 59.343 | 60.028 | End: 242 |
| Size | 20 | 22 | 100 |
| Primer 2 details |
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| Sequence | TTTTACACACGCGGTTCCAAC | TCCGCATTTATATCACTCGGGG | Start: 142 |
| Tm | 59.936 | 60.028 | End: 242 |
| Size | 21 | 22 | 101 |
| Primer 3 details |
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| Sequence | TTTTTACACACGCGGTTCCA | CGCATTTATATCACTCGGGGC | Start: 141 |
| Tm | 58.623 | 59.268 | End: 240 |
| Size | 20 | 21 | 100 |
| Primer 4 details |
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| Sequence | TGTGCCGGAGACTACAAAGC | TCTGATAGAAGAACACCGGGT | Start: 192 |
| Tm | 60.32 | 58.184 | End: 349 |
| Size | 20 | 21 | 158 |
| Primer 5 details |
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| Sequence | GGAGACTACAAAGCGCCCAA | TCTGATAGAAGAACACCGGGT | Start: 198 |
| Tm | 60.321 | 58.184 | End: 349 |
| Size | 20 | 21 | 152 |
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