ssr_primer
Primer Details for TRINITY_DN66469_c4_g3_i1 |
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| (T)12gtcagaaatagatatacaaggctattaaaattgagacaccagtgcacttagaaaaatgcaattcaattaatgtagtgcatacttcagga(G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATAAATTCCCCGGGTCGC | CCCCCTCCTGAAGTATGCAC | Start: 17 |
| Tm | 59.892 | 59.819 | End: 226 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
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| Sequence | TGCAGCGCAATTATCAGCTC | CCCCCTCCTGAAGTATGCAC | Start: 45 |
| Tm | 59.341 | 59.819 | End: 226 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
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| Sequence | GCGGCCGCAGAGAAGATAAA | CCCCCTCCTGAAGTATGCAC | Start: 4 |
| Tm | 60.81 | 59.819 | End: 226 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
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| Sequence | GATAAATTCCCCGGGTCGCC | CCCCCTCCTGAAGTATGCAC | Start: 18 |
| Tm | 60.888 | 59.819 | End: 226 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
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| Sequence | GCAGCGCAATTATCAGCTCT | CCCCCTCCTGAAGTATGCAC | Start: 46 |
| Tm | 59.057 | 59.819 | End: 226 |
| Size | 20 | 20 | 181 |
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