ssr_primer
Primer Details for TRINITY_DN66462_c2_g1_i11 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGTGCCTGCGCTGTAC | TAAATCCGGCCCTGGGTAGA | Start: 1481 |
| Tm | 59.97 | 60.031 | End: 1718 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TTTTTGTGCCTGCGCTGTAC | GTAAATCCGGCCCTGGGTAG | Start: 1481 |
| Tm | 59.97 | 59.892 | End: 1719 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | GCGGGACTTGAGGAAGAGAC | GTACAGCGCAGGCACAAAAA | Start: 1257 |
| Tm | 60.109 | 59.97 | End: 1500 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GTTAGGTGCCCTATGCCGAA | TAAATCCGGCCCTGGGTAGA | Start: 1618 |
| Tm | 59.821 | 60.031 | End: 1718 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AAGTTAGGTGCCCTATGCCG | TAAATCCGGCCCTGGGTAGA | Start: 1616 |
| Tm | 59.82 | 60.031 | End: 1718 |
| Size | 20 | 20 | 103 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGTGCCTGCGCTGTAC | TAAATCCGGCCCTGGGTAGA | Start: 1481 |
| Tm | 59.97 | 60.031 | End: 1718 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TTTTTGTGCCTGCGCTGTAC | GTAAATCCGGCCCTGGGTAG | Start: 1481 |
| Tm | 59.97 | 59.892 | End: 1719 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | GCGGGACTTGAGGAAGAGAC | GTACAGCGCAGGCACAAAAA | Start: 1257 |
| Tm | 60.109 | 59.97 | End: 1500 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GTTAGGTGCCCTATGCCGAA | TAAATCCGGCCCTGGGTAGA | Start: 1618 |
| Tm | 59.821 | 60.031 | End: 1718 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AAGTTAGGTGCCCTATGCCG | TAAATCCGGCCCTGGGTAGA | Start: 1616 |
| Tm | 59.82 | 60.031 | End: 1718 |
| Size | 20 | 20 | 103 |
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