ssr_primer
Primer Details for TRINITY_DN66454_c3_g3_i1 |
|---|
| (T)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGTAATCGGCCCGAAAC | AACCGCATTTCGTTGGTTGG | Start: 157 |
| Tm | 59.971 | 59.969 | End: 283 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TTTGATCGTGAGACCCAGGC | CCACTTGCACTCGTGGTTTG | Start: 609 |
| Tm | 60.036 | 59.971 | End: 789 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | GTGAGACCCAGGCTTCAGAC | CCACTTGCACTCGTGGTTTG | Start: 616 |
| Tm | 60.037 | 59.971 | End: 789 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | CCAACCAACGAAATGCGGTT | TGTGATCCAGGCTCAACGAC | Start: 264 |
| Tm | 59.969 | 60.037 | End: 474 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | CCAACCAACGAAATGCGGTT | GTGTGATCCAGGCTCAACGA | Start: 264 |
| Tm | 59.969 | 60.037 | End: 475 |
| Size | 20 | 20 | 212 |
(T)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGTAATCGGCCCGAAAC | AACCGCATTTCGTTGGTTGG | Start: 157 |
| Tm | 59.971 | 59.969 | End: 283 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | TTTGATCGTGAGACCCAGGC | CCACTTGCACTCGTGGTTTG | Start: 609 |
| Tm | 60.036 | 59.971 | End: 789 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | GTGAGACCCAGGCTTCAGAC | CCACTTGCACTCGTGGTTTG | Start: 616 |
| Tm | 60.037 | 59.971 | End: 789 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | CCAACCAACGAAATGCGGTT | TGTGATCCAGGCTCAACGAC | Start: 264 |
| Tm | 59.969 | 60.037 | End: 474 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | CCAACCAACGAAATGCGGTT | GTGTGATCCAGGCTCAACGA | Start: 264 |
| Tm | 59.969 | 60.037 | End: 475 |
| Size | 20 | 20 | 212 |
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