ssr_primer
Primer Details for TRINITY_DN66453_c5_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGATGGAGGGCGCTCT | CGTGCGCCCCTCTAAAATTG | Start: 349 |
| Tm | 59.962 | 59.901 | End: 547 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
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| Sequence | TTTTTGATGGAGGGCGCTCT | GTGCGCCCCTCTAAAATTGC | Start: 349 |
| Tm | 59.962 | 60.179 | End: 546 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | TTTTTGATGGAGGGCGCTCT | CTCCAGAACACGAGAGAGCG | Start: 349 |
| Tm | 59.962 | 60.179 | End: 614 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
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| Sequence | GTTTTTGATGGAGGGCGCTC | CGTGCGCCCCTCTAAAATTG | Start: 348 |
| Tm | 59.829 | 59.901 | End: 547 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | GGGCGCTCTCTAATGTTCCA | CGTGCGCCCCTCTAAAATTG | Start: 360 |
| Tm | 59.822 | 59.901 | End: 547 |
| Size | 20 | 20 | 188 |
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