ssr_primer
Primer Details for TRINITY_DN66453_c4_g1_i1 |
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| (C)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAATTCGCGATACCGGA | TTGAGTCGAACCGTCTTGGG | Start: 370 |
| Tm | 59.968 | 59.967 | End: 552 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | CCAGAATTCGCGATACCGGA | CCGTCTTGGGCTTGTAGTGT | Start: 370 |
| Tm | 59.968 | 59.965 | End: 542 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
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| Sequence | CCAGAATTCGCGATACCGGA | ACCGTCTTGGGCTTGTAGTG | Start: 370 |
| Tm | 59.968 | 59.965 | End: 543 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
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| Sequence | TGGTGCGGGCTGGAAATTAT | TTGAGTCGAACCGTCTTGGG | Start: 293 |
| Tm | 60.034 | 59.967 | End: 552 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
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| Sequence | TGGTGCGGGCTGGAAATTAT | CCGTCTTGGGCTTGTAGTGT | Start: 293 |
| Tm | 60.034 | 59.965 | End: 542 |
| Size | 20 | 20 | 250 |
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