ssr_primer
Primer Details for TRINITY_DN66416_c4_g1_i2 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAAACCCTCAAGACCCCC | GCAGAATGTGGGAAAGTCGC | Start: 158 |
| Tm | 59.962 | 59.831 | End: 301 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | CGTTTTGGGGTGGTTCCAAC | GCAGAATGTGGGAAAGTCGC | Start: 97 |
| Tm | 59.897 | 59.831 | End: 301 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | GAAACCCTCAAGACCCCCAC | GCAGAATGTGGGAAAGTCGC | Start: 160 |
| Tm | 60.251 | 59.831 | End: 301 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | CAACCCCCTCTTTGACCAGG | GCAGAATGTGGGAAAGTCGC | Start: 113 |
| Tm | 60.251 | 59.831 | End: 301 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
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| Sequence | CCAACCCCCTCTTTGACCAG | GCAGAATGTGGGAAAGTCGC | Start: 112 |
| Tm | 60.251 | 59.831 | End: 301 |
| Size | 20 | 20 | 190 |
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