ssr_primer
Primer Details for TRINITY_DN66345_c5_g5_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCATGCTGTGTTGAGGGG | GCTCCACCCTACATTGGCAT | Start: 2394 |
| Tm | 60.034 | 60.107 | End: 2617 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
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| Sequence | ATCCATGCTGTGTTGAGGGG | TGAACAACCATGCTCTGCCT | Start: 2394 |
| Tm | 60.034 | 59.889 | End: 2573 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
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| Sequence | ATGCCAATGTAGGGTGGAGC | TTTGCTCCCCAAGGTCCATC | Start: 2598 |
| Tm | 60.107 | 59.96 | End: 2722 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
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| Sequence | GTGTTTGCCGGGTTGCATAG | TTTGCTCCCCAAGGTCCATC | Start: 2519 |
| Tm | 60.109 | 59.96 | End: 2722 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | AGGCAGAGCATGGTTGTTCA | TTTGCTCCCCAAGGTCCATC | Start: 2554 |
| Tm | 59.889 | 59.96 | End: 2722 |
| Size | 20 | 20 | 169 |
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