ssr_primer
Primer Details for TRINITY_DN66345_c3_g2_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTGCTGTTTACCGGTCG | ACGAGAGACATGGTTTGGGA | Start: 326 |
| Tm | 60.039 | 58.652 | End: 455 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | GAGTGCTGTTTACCGGTCGA | ACGAGAGACATGGTTTGGGA | Start: 327 |
| Tm | 60.039 | 58.652 | End: 455 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | AAGCTCATGAGGTGCCACAA | ACGAGAGACATGGTTTGGGA | Start: 351 |
| Tm | 59.889 | 58.652 | End: 455 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | AAAGCTCATGAGGTGCCACA | ACGAGAGACATGGTTTGGGA | Start: 350 |
| Tm | 59.889 | 58.652 | End: 455 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
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| Sequence | AGAGTGCTGTTTACCGGTCG | AGCTCAAACGAGAGACATGGT | Start: 326 |
| Tm | 60.039 | 59.379 | End: 462 |
| Size | 20 | 21 | 137 |
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