Primer Details for TRINITY_DN66340_c3_g1_i1 |
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| (A)17caacacag(A)11ggaaaaacaaacagaagagagatcaacgagaaac(A)11tgcaaaaaagaaaggtaaaaaagaactagggaaaaaagcaaaaagagatgagaagaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGAAGAGAGATCAACGAGAAACA | TCTCCTCTTATCCCTCTAGTTTCT | Start: 81 |
| Tm | 59.99 | 57.4 | End: 207 |
| Size | 25 | 24 | 127 |
| Primer 2 details |
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| Sequence | ACAAACAGAAGAGAGATCAACGAGA | TCTCCTCTTATCCCTCTAGTTTCT | Start: 77 |
| Tm | 59.99 | 57.4 | End: 207 |
| Size | 25 | 24 | 131 |
| Primer 3 details |
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| Sequence | ACAAACAGAAGAGAGATCAACGA | TCTCCTCTTATCCCTCTAGTTTCT | Start: 77 |
| Tm | 57.798 | 57.4 | End: 207 |
| Size | 23 | 24 | 131 |
| Primer 4 details |
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| Sequence | ACAGAAGAGAGATCAACGAGAAACA | TCTGTATCTCCTCTTATCCCTCTAGT | Start: 81 |
| Tm | 59.99 | 59.204 | End: 213 |
| Size | 25 | 26 | 133 |
| Primer 5 details |
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| Sequence | ACAAACAGAAGAGAGATCAACGAGA | TCTGTATCTCCTCTTATCCCTCTAGT | Start: 77 |
| Tm | 59.99 | 59.204 | End: 213 |
| Size | 25 | 26 | 137 |
