ssr_primer
Primer Details for TRINITY_DN66309_c7_g3_i1 |
|---|
| (T)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGAATTTGCGGCATTTGGA | TACTCATCGACCCACGGACT | Start: 440 |
| Tm | 60.109 | 60.035 | End: 590 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCGGAATTTGCGGCATTTGG | TACTCATCGACCCACGGACT | Start: 439 |
| Tm | 60.109 | 60.035 | End: 590 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | AGCCAGGCCGAAAATCGTAA | CCAAATGCCGCAAATTCCGA | Start: 270 |
| Tm | 60.036 | 60.109 | End: 458 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | AGCCAGGCCGAAAATCGTAA | TCCAAATGCCGCAAATTCCG | Start: 270 |
| Tm | 60.036 | 60.109 | End: 459 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | CGGAATTTGCGGCATTTGGA | CCCACGGACTTCATTTCCCA | Start: 440 |
| Tm | 60.109 | 59.962 | End: 580 |
| Size | 20 | 20 | 141 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGAATTTGCGGCATTTGGA | TACTCATCGACCCACGGACT | Start: 440 |
| Tm | 60.109 | 60.035 | End: 590 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCGGAATTTGCGGCATTTGG | TACTCATCGACCCACGGACT | Start: 439 |
| Tm | 60.109 | 60.035 | End: 590 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | AGCCAGGCCGAAAATCGTAA | CCAAATGCCGCAAATTCCGA | Start: 270 |
| Tm | 60.036 | 60.109 | End: 458 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | AGCCAGGCCGAAAATCGTAA | TCCAAATGCCGCAAATTCCG | Start: 270 |
| Tm | 60.036 | 60.109 | End: 459 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | CGGAATTTGCGGCATTTGGA | CCCACGGACTTCATTTCCCA | Start: 440 |
| Tm | 60.109 | 59.962 | End: 580 |
| Size | 20 | 20 | 141 |
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