ssr_primer
Primer Details for TRINITY_DN66290_c4_g3_i7 |
|---|
| (A)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTAAAGCGGATGCCTCTC | AAGATCCAAACCCTGCGGAG | Start: 55 |
| Tm | 60.179 | 60.035 | End: 195 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TGCTAAAGCGGATGCCTCTC | GGAACGGATGAACGGAGAGG | Start: 55 |
| Tm | 60.179 | 60.179 | End: 280 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | AGCGGATGCCTCTCCTTTTT | AAGATCCAAACCCTGCGGAG | Start: 61 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | AAGCGGATGCCTCTCCTTTT | AAGATCCAAACCCTGCGGAG | Start: 60 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | AAAGCGGATGCCTCTCCTTT | AAGATCCAAACCCTGCGGAG | Start: 59 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 137 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTAAAGCGGATGCCTCTC | AAGATCCAAACCCTGCGGAG | Start: 55 |
| Tm | 60.179 | 60.035 | End: 195 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TGCTAAAGCGGATGCCTCTC | GGAACGGATGAACGGAGAGG | Start: 55 |
| Tm | 60.179 | 60.179 | End: 280 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | AGCGGATGCCTCTCCTTTTT | AAGATCCAAACCCTGCGGAG | Start: 61 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | AAGCGGATGCCTCTCCTTTT | AAGATCCAAACCCTGCGGAG | Start: 60 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | AAAGCGGATGCCTCTCCTTT | AAGATCCAAACCCTGCGGAG | Start: 59 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 137 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTAAAGCGGATGCCTCTC | AAGATCCAAACCCTGCGGAG | Start: 55 |
| Tm | 60.179 | 60.035 | End: 195 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | TGCTAAAGCGGATGCCTCTC | GGAACGGATGAACGGAGAGG | Start: 55 |
| Tm | 60.179 | 60.179 | End: 280 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | AGCGGATGCCTCTCCTTTTT | AAGATCCAAACCCTGCGGAG | Start: 61 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | AAGCGGATGCCTCTCCTTTT | AAGATCCAAACCCTGCGGAG | Start: 60 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | AAAGCGGATGCCTCTCCTTT | AAGATCCAAACCCTGCGGAG | Start: 59 |
| Tm | 59.67 | 60.035 | End: 195 |
| Size | 20 | 20 | 137 |
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