ssr_primer
Primer Details for TRINITY_DN66278_c3_g1_i3 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAGCACCAACACAACG | TGAACACACCCTCTTGTCGG | Start: 1726 |
| Tm | 59.971 | 59.894 | End: 1965 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | CCAGAAGCACCAACACAACG | GGTGCTTCTGGGGAGGAAAA | Start: 1591 |
| Tm | 59.971 | 59.888 | End: 1736 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCCTCCCCAGAAGCACC | CGTTGTGTTGGTGCTTCTGG | Start: 1582 |
| Tm | 59.888 | 59.971 | End: 1745 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | TTTTCCTCCCCAGAAGCACC | TGAACACACCCTCTTGTCGG | Start: 1717 |
| Tm | 59.888 | 59.894 | End: 1965 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | CCAGAAGCACCAACACAACG | AGGGTTGTACGAGGGTGGAT | Start: 1726 |
| Tm | 59.971 | 60.253 | End: 1878 |
| Size | 20 | 20 | 153 |
(A)23caggttacaaag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAGCACCAACACAACG | TGAACACACCCTCTTGTCGG | Start: 1726 |
| Tm | 59.971 | 59.894 | End: 1965 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | CCAGAAGCACCAACACAACG | GGTGCTTCTGGGGAGGAAAA | Start: 1591 |
| Tm | 59.971 | 59.888 | End: 1736 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCCTCCCCAGAAGCACC | CGTTGTGTTGGTGCTTCTGG | Start: 1582 |
| Tm | 59.888 | 59.971 | End: 1745 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | TTTTCCTCCCCAGAAGCACC | TGAACACACCCTCTTGTCGG | Start: 1717 |
| Tm | 59.888 | 59.894 | End: 1965 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | CCAGAAGCACCAACACAACG | AGGGTTGTACGAGGGTGGAT | Start: 1726 |
| Tm | 59.971 | 60.253 | End: 1878 |
| Size | 20 | 20 | 153 |
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