ssr_primer
Primer Details for TRINITY_DN66254_c5_g4_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGAAGTTGACGGCCAAGC | GATAGGCTTGCACATTGGCG | Start: 1768 |
| Tm | 60.04 | 59.97 | End: 2036 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
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| Sequence | GCAACCTTTTGGAAGCGAGG | GCTTGGCCGTCAACTTCTTG | Start: 1673 |
| Tm | 60.039 | 60.04 | End: 1787 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
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| Sequence | CTGAGAGTTGACCGGCTCTG | CCTCGCTTCCAAAAGGTTGC | Start: 1431 |
| Tm | 60.109 | 60.039 | End: 1692 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
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| Sequence | GAGAGTTGACCGGCTCTGAG | CCTCGCTTCCAAAAGGTTGC | Start: 1433 |
| Tm | 59.828 | 60.039 | End: 1692 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
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| Sequence | GACCGGCTCTGAGCAAACTA | CCTCGCTTCCAAAAGGTTGC | Start: 1440 |
| Tm | 59.753 | 60.039 | End: 1692 |
| Size | 20 | 20 | 253 |
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