ssr_primer
Primer Details for TRINITY_DN66254_c4_g2_i3 |
|---|
| (TACA)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCAATGGACGGGTCGTAA | GGAGGGAACAAGCTCACACA | Start: 203 |
| Tm | 59.965 | 59.891 | End: 350 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGGGGTCCAAAGACG | CGTGTTCGCTCTCCAACTCT | Start: 929 |
| Tm | 59.89 | 60.039 | End: 1159 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCAATGGACGGGTCGTAA | TTTTTGAGCCAACGTGCGTT | Start: 203 |
| Tm | 59.965 | 59.829 | End: 402 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | CACCTCGCCACTCTGTTAGG | AGACGTGAAAGCCCATCTCG | Start: 665 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCACCTCGCCACTCTGTTAG | AGACGTGAAAGCCCATCTCG | Start: 664 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 136 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCAATGGACGGGTCGTAA | GGAGGGAACAAGCTCACACA | Start: 203 |
| Tm | 59.965 | 59.891 | End: 350 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGGGGTCCAAAGACG | CGTGTTCGCTCTCCAACTCT | Start: 929 |
| Tm | 59.89 | 60.039 | End: 1159 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCAATGGACGGGTCGTAA | TTTTTGAGCCAACGTGCGTT | Start: 203 |
| Tm | 59.965 | 59.829 | End: 402 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | CACCTCGCCACTCTGTTAGG | AGACGTGAAAGCCCATCTCG | Start: 665 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCACCTCGCCACTCTGTTAG | AGACGTGAAAGCCCATCTCG | Start: 664 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 136 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCAATGGACGGGTCGTAA | GGAGGGAACAAGCTCACACA | Start: 203 |
| Tm | 59.965 | 59.891 | End: 350 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | TTTCAGGGGGTCCAAAGACG | CGTGTTCGCTCTCCAACTCT | Start: 929 |
| Tm | 59.89 | 60.039 | End: 1159 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTGCAATGGACGGGTCGTAA | TTTTTGAGCCAACGTGCGTT | Start: 203 |
| Tm | 59.965 | 59.829 | End: 402 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | CACCTCGCCACTCTGTTAGG | AGACGTGAAAGCCCATCTCG | Start: 665 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCACCTCGCCACTCTGTTAG | AGACGTGAAAGCCCATCTCG | Start: 664 |
| Tm | 60.109 | 60.109 | End: 799 |
| Size | 20 | 20 | 136 |
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