Primer Details for TRINITY_DN66226_c6_g1_i1 |
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| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTTTAGACGTTGCCGAA | TGGGTTTTTATTGTTTTTCAGGAGAGT | Start: 47 |
| Tm | 59.966 | 59.875 | End: 215 |
| Size | 20 | 27 | 169 |
| Primer 2 details |
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| Sequence | AGCCTTTAGACGTTGCCGAA | AGTGGGTTTTTATTGTTTTTCAGGAGA | Start: 47 |
| Tm | 59.966 | 59.875 | End: 217 |
| Size | 20 | 27 | 171 |
| Primer 3 details |
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| Sequence | AAACGCCGGATGAGCCTTTA | TGGGTTTTTATTGTTTTTCAGGAGAGT | Start: 35 |
| Tm | 60.036 | 59.875 | End: 215 |
| Size | 20 | 27 | 181 |
| Primer 4 details |
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| Sequence | AAACGCCGGATGAGCCTTTA | AGTGGGTTTTTATTGTTTTTCAGGAGA | Start: 35 |
| Tm | 60.036 | 59.875 | End: 217 |
| Size | 20 | 27 | 183 |
| Primer 5 details |
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| Sequence | AGCCTTTAGACGTTGCCGAA | AGTGGGTTTTTATTGTTTTTCAGGA | Start: 47 |
| Tm | 59.966 | 57.811 | End: 217 |
| Size | 20 | 25 | 171 |
