ssr_primer
Primer Details for TRINITY_DN66191_c6_g3_i2 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATAGAACTTTGCGCTCCG | TCCATGTTGGTCACCTGCTC | Start: 461 |
| Tm | 59.973 | 59.963 | End: 731 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
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| Sequence | CACTTTCGCAGTCACTTGGC | CGGAGCGCAAAGTTCTATGC | Start: 249 |
| Tm | 60.041 | 59.973 | End: 480 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | GAGCAGGTGACCAACATGGA | TGGCGTCCAACAACTCTCTC | Start: 712 |
| Tm | 59.963 | 59.966 | End: 900 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
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| Sequence | GAGCAGGTGACCAACATGGA | TTGCAACATTTCCTGCTGGC | Start: 712 |
| Tm | 59.963 | 59.966 | End: 960 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
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| Sequence | CATTGTCTAGCCCCTGCCAA | GCCAAGTGACTGCGAAAGTG | Start: 6 |
| Tm | 60.034 | 60.041 | End: 268 |
| Size | 20 | 20 | 263 |
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