ssr_primer
Primer Details for TRINITY_DN66176_c2_g2_i2 |
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| (TTAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTTGCGCACTAATCCCA | ATGGTTGGCCTCTGAGTGTG | Start: 2998 |
| Tm | 59.964 | 59.963 | End: 3218 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
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| Sequence | ACTGTTGCGCACTAATCCCA | CATGGTTGGCCTCTGAGTGT | Start: 2998 |
| Tm | 59.964 | 59.963 | End: 3219 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
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| Sequence | AAGGTGTTCCTGTGGTTGGG | CTGAGTCCGTCCGTCTGATG | Start: 53 |
| Tm | 60.106 | 59.9 | End: 321 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
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| Sequence | CGTTGCTCATCTTTGGCAGG | TGGGATTAGTGCGCAACAGT | Start: 2871 |
| Tm | 59.83 | 59.964 | End: 3017 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | GGTGTTCCTGTGGTTGGGAA | CTGAGTCCGTCCGTCTGATG | Start: 55 |
| Tm | 60.106 | 59.9 | End: 321 |
| Size | 20 | 20 | 267 |
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