ssr_primer
Primer Details for TRINITY_DN66157_c0_g1_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCCAGAACTGATGCCTT | GGGGCAAGGTGTCATAAGCT | Start: 447 |
| Tm | 60.035 | 60.034 | End: 720 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGAGCCAGAACTGATGCCTT | AGGGGCAAGGTGTCATAAGC | Start: 447 |
| Tm | 60.035 | 60.034 | End: 721 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | TTCACCTCAGCAAGCTCCTG | GACATCGCCCTCTTTGGACA | Start: 2252 |
| Tm | 59.964 | 60.036 | End: 2432 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | ACCTTCACCTCAGCAAGCTC | GACATCGCCCTCTTTGGACA | Start: 2249 |
| Tm | 59.964 | 60.036 | End: 2432 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TGCTCGAACCTTTGGCAGAT | GACATCGCCCTCTTTGGACA | Start: 2275 |
| Tm | 59.963 | 60.036 | End: 2432 |
| Size | 20 | 20 | 158 |
(TC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCCAGAACTGATGCCTT | GGGGCAAGGTGTCATAAGCT | Start: 447 |
| Tm | 60.035 | 60.034 | End: 720 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGAGCCAGAACTGATGCCTT | AGGGGCAAGGTGTCATAAGC | Start: 447 |
| Tm | 60.035 | 60.034 | End: 721 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | TTCACCTCAGCAAGCTCCTG | GACATCGCCCTCTTTGGACA | Start: 2252 |
| Tm | 59.964 | 60.036 | End: 2432 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | ACCTTCACCTCAGCAAGCTC | GACATCGCCCTCTTTGGACA | Start: 2249 |
| Tm | 59.964 | 60.036 | End: 2432 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TGCTCGAACCTTTGGCAGAT | GACATCGCCCTCTTTGGACA | Start: 2275 |
| Tm | 59.963 | 60.036 | End: 2432 |
| Size | 20 | 20 | 158 |
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