ssr_primer
Primer Details for TRINITY_DN66128_c3_g1_i1 |
|---|
| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATTAAGGGAGCCCACGGG | TGGTCCACACGAAAAGAGCT | Start: 436 |
| Tm | 60.034 | 59.532 | End: 637 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
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| Sequence | ACCGGGTTGATGCAGGAATT | TGGTCCACACGAAAAGAGCT | Start: 384 |
| Tm | 59.96 | 59.532 | End: 637 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
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| Sequence | TTTGAGACCGGGTTGATGCA | TGGTCCACACGAAAAGAGCT | Start: 378 |
| Tm | 59.89 | 59.532 | End: 637 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | CGGAAAAGGTTTGGCAACGT | AAGTGCTGTGAGGCATGACC | Start: 52 |
| Tm | 59.898 | 60.607 | End: 284 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | TTGAGACCGGGTTGATGCAG | TGGTCCACACGAAAAGAGCT | Start: 379 |
| Tm | 60.322 | 59.532 | End: 637 |
| Size | 20 | 20 | 259 |
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