ssr_primer
Primer Details for TRINITY_DN66111_c0_g5_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGCACATGTAGGCCAAGG | ATGCGCTCCTGAGGAATAGC | Start: 158 |
| Tm | 60.108 | 59.965 | End: 260 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | AGGTGTCTTGTTCGTGTGCA | ATGCGCTCCTGAGGAATAGC | Start: 20 |
| Tm | 60.108 | 59.965 | End: 260 |
| Size | 20 | 20 | 241 |
| Primer 3 details |
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| Sequence | AGGTGTCTTGTTCGTGTGCA | CCTTGGCCTACATGTGCGTA | Start: 20 |
| Tm | 60.108 | 60.108 | End: 177 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
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| Sequence | AGGTGTCTTGTTCGTGTGCA | GTTGCCTGGTCGTCGGATAA | Start: 20 |
| Tm | 60.108 | 60.109 | End: 223 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | ATGACAGGAAAGCCCCGTTT | ACAATGTCGCCCATCCGAAT | Start: 361 |
| Tm | 59.888 | 60.107 | End: 583 |
| Size | 20 | 20 | 223 |
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