ssr_primer
Primer Details for TRINITY_DN66106_c4_g2_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAGTGACAATTGGTTTTGGC | TGAAGGCAAGACAGGACTGA | Start: 50 |
| Tm | 59.128 | 58.576 | End: 327 |
| Size | 22 | 20 | 278 |
| Primer 2 details |
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| Sequence | CCAAGTGACAATTGGTTTTGGC | TTGAAGGCAAGACAGGACTGA | Start: 50 |
| Tm | 59.128 | 59.232 | End: 328 |
| Size | 22 | 21 | 279 |
| Primer 3 details |
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| Sequence | CCAAGTGACAATTGGTTTTGGC | TGAAGGCAAGACAGGACTGAT | Start: 50 |
| Tm | 59.128 | 59.019 | End: 327 |
| Size | 22 | 21 | 278 |
| Primer 4 details |
|---|
| Sequence | TCCAAGTGACAATTGGTTTTGGC | TGAAGGCAAGACAGGACTGA | Start: 49 |
| Tm | 60.431 | 58.576 | End: 327 |
| Size | 23 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | CAAGTGACAATTGGTTTTGGCA | TGAAGGCAAGACAGGACTGA | Start: 51 |
| Tm | 58.47 | 58.576 | End: 327 |
| Size | 22 | 20 | 277 |
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