ssr_primer
Primer Details for TRINITY_DN66097_c5_g3_i3 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGGGTGTGCTCGTTAGTGG | AAGGAGCATTGTGGACAGGG | Start: 48 |
| Tm | 59.965 | 59.961 | End: 181 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
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| Sequence | AAGGGTGTGCTCGTTAGTGG | TGGAACTTAGCGCCAACTGT | Start: 48 |
| Tm | 59.965 | 59.892 | End: 231 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
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| Sequence | ATAAAGCGCCAGTGACGTCA | GACGAGTGGGAAAGATGGGG | Start: 90 |
| Tm | 60.038 | 60.108 | End: 342 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
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| Sequence | ATAAAGCGCCAGTGACGTCA | TGGAACTTAGCGCCAACTGT | Start: 90 |
| Tm | 60.038 | 59.892 | End: 231 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
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| Sequence | CCCTGTCCACAATGCTCCTT | GACGAGTGGGAAAGATGGGG | Start: 162 |
| Tm | 59.961 | 60.108 | End: 342 |
| Size | 20 | 20 | 181 |
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