ssr_primer
Primer Details for TRINITY_DN66079_c9_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCTCTCTGTGCGAGGA | GACGACCTCAGAGCACAACA | Start: 2232 |
| Tm | 59.963 | 59.968 | End: 2425 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCAGTCTCTCTGTGCGAGGA | TGACGACCTCAGAGCACAAC | Start: 2232 |
| Tm | 59.963 | 59.968 | End: 2426 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | GGATGACGTTGCTCTGTCCA | GACGACCTCAGAGCACAACA | Start: 2249 |
| Tm | 60.037 | 59.968 | End: 2425 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | GGATGACGTTGCTCTGTCCA | TGACGACCTCAGAGCACAAC | Start: 2249 |
| Tm | 60.037 | 59.968 | End: 2426 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | GGGTTCGCAAGTGCAATTGT | GGACTCATGCCCCAAACTCA | Start: 2797 |
| Tm | 59.968 | 59.962 | End: 3034 |
| Size | 20 | 20 | 238 |
(A)11tcaaatcccttgaaaagtaaaattacagaaagatgatgcttcaaaagac(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCTCTCTGTGCGAGGA | GACGACCTCAGAGCACAACA | Start: 2232 |
| Tm | 59.963 | 59.968 | End: 2425 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCAGTCTCTCTGTGCGAGGA | TGACGACCTCAGAGCACAAC | Start: 2232 |
| Tm | 59.963 | 59.968 | End: 2426 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | GGATGACGTTGCTCTGTCCA | GACGACCTCAGAGCACAACA | Start: 2249 |
| Tm | 60.037 | 59.968 | End: 2425 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | GGATGACGTTGCTCTGTCCA | TGACGACCTCAGAGCACAAC | Start: 2249 |
| Tm | 60.037 | 59.968 | End: 2426 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | GGGTTCGCAAGTGCAATTGT | GGACTCATGCCCCAAACTCA | Start: 2797 |
| Tm | 59.968 | 59.962 | End: 3034 |
| Size | 20 | 20 | 238 |
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