ssr_primer
Primer Details for TRINITY_DN66075_c3_g1_i3 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAACAAGGTGCACACGACC | GGGTTCTTGTGAGCTCTGCT | Start: 188 |
| Tm | 59.971 | 59.964 | End: 366 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
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| Sequence | GAAACAAGGTGCACACGACC | AGGGTTCTTGTGAGCTCTGC | Start: 188 |
| Tm | 59.971 | 59.964 | End: 367 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
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| Sequence | GAAACAAGGTGCACACGACC | TGCCAGCAATGACAGTGGAT | Start: 188 |
| Tm | 59.971 | 59.961 | End: 346 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | GTGGAATCCTCGCAAGGGAA | TCGAAGAAGGAGCCTCCGTA | Start: 715 |
| Tm | 60.035 | 60.035 | End: 933 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
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| Sequence | AGCTTACGTTTTGCAAGCCG | GGCCGAGCAGCATGTATTTG | Start: 1263 |
| Tm | 60.04 | 59.97 | End: 1531 |
| Size | 20 | 20 | 269 |
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