ssr_primer
Primer Details for TRINITY_DN66062_c3_g4_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACTTGCATCTCCGCTAGGT | AGGATGAGATCTTCGATGTGCA | Start: 63 |
| Tm | 59.823 | 59.303 | End: 233 |
| Size | 20 | 22 | 171 |
| Primer 2 details |
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| Sequence | CACTTGCATCTCCGCTAGGT | TCTGATTGTGGCATCATGGT | Start: 63 |
| Tm | 59.823 | 57.187 | End: 199 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | TCCGCTAGGTTTTGAACGGT | AGGATGAGATCTTCGATGTGCA | Start: 73 |
| Tm | 59.606 | 59.303 | End: 233 |
| Size | 20 | 22 | 161 |
| Primer 4 details |
|---|
| Sequence | TCCGCTAGGTTTTGAACGGT | TCTGATTGTGGCATCATGGT | Start: 73 |
| Tm | 59.606 | 57.187 | End: 199 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | CACTTGCATCTCCGCTAGGT | TCTGATTGTGGCATCATGGTT | Start: 63 |
| Tm | 59.823 | 57.91 | End: 199 |
| Size | 20 | 21 | 137 |
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