ssr_primer
Primer Details for TRINITY_DN66053_c11_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGTGCGGTAAAGCCTAGTT | GAGAAGGAAAAGCGGGGTGA | Start: 1719 |
| Tm | 60.036 | 59.964 | End: 1944 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
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| Sequence | GGGTGCGGTAAAGCCTAGTT | AGAGAAGGAAAAGCGGGGTG | Start: 1719 |
| Tm | 60.036 | 59.963 | End: 1945 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | TCACCCCGCTTTTCCTTCTC | ACAGTAGGGCGAAATTGGCA | Start: 1925 |
| Tm | 59.964 | 59.962 | End: 2059 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | CACCCCGCTTTTCCTTCTCT | ACAGTAGGGCGAAATTGGCA | Start: 1926 |
| Tm | 59.963 | 59.962 | End: 2059 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | CGCACCGGCTTAAGGACTTA | GGCGATGCCGGATTTTTACC | Start: 1256 |
| Tm | 60.109 | 59.97 | End: 1385 |
| Size | 20 | 20 | 130 |
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