Primer Details for TRINITY_DN66013_c4_g7_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTAGTTGTACCGACAGA | AAAAGATCGATGTACAAATGGGTT | Start: 128 |
| Tm | 59.477 | 57.166 | End: 229 |
| Size | 20 | 24 | 102 |
| Primer 2 details | |||
| Sequence | GAGCCGTAGTTGTACCGACA | AAAAGATCGATGTACAAATGGGTT | Start: 126 |
| Tm | 59.477 | 57.166 | End: 229 |
| Size | 20 | 24 | 104 |
| Primer 3 details | |||
| Sequence | TGAGCCGTAGTTGTACCGAC | AAAAGATCGATGTACAAATGGGTT | Start: 125 |
| Tm | 59.477 | 57.166 | End: 229 |
| Size | 20 | 24 | 105 |
| Primer 4 details | |||
| Sequence | AGCCGTAGTTGTACCGACAG | AAAAGATCGATGTACAAATGGGTT | Start: 127 |
| Tm | 59.475 | 57.166 | End: 229 |
| Size | 20 | 24 | 103 |
| Primer 5 details | |||
| Sequence | GCCGTAGTTGTACCGACAGA | TCTAAAAGATCGATGTACAAATGGGT | Start: 128 |
| Tm | 59.477 | 58.664 | End: 232 |
| Size | 20 | 26 | 105 |
