ssr_primer
Primer Details for TRINITY_DN65971_c4_g1_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGACCCAGAAAAGCGCACT | TCGACTAACAAGCACACCCC | Start: 272 |
| Tm | 60.179 | 59.966 | End: 399 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | AAGACCCAGAAAAGCGCACT | CGACTAACAAGCACACCCCT | Start: 272 |
| Tm | 60.179 | 59.965 | End: 398 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | AAGACCCAGAAAAGCGCACT | ACTGCCCTCGACTAACAAGC | Start: 272 |
| Tm | 60.179 | 60.037 | End: 406 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | AAGACCCAGAAAAGCGCACT | CTGCCCTCGACTAACAAGCA | Start: 272 |
| Tm | 60.179 | 60.037 | End: 405 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | AAGACCCAGAAAAGCGCACT | GATTTCGACAACGGCGAAGG | Start: 272 |
| Tm | 60.179 | 59.905 | End: 475 |
| Size | 20 | 20 | 204 |
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