ssr_primer
Primer Details for TRINITY_DN65956_c5_g3_i1 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCTCAAATTAAGAGGCTTGG | CGGTGATAGCCGCAACAATT | Start: 45 |
| Tm | 59.902 | 59.267 | End: 219 |
| Size | 22 | 20 | 175 |
| Primer 2 details |
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| Sequence | CGCTCAAATTAAGAGGCTTGGT | CGGTGATAGCCGCAACAATT | Start: 46 |
| Tm | 59.25 | 59.267 | End: 219 |
| Size | 22 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | TCCGCTCAAATTAAGAGGCTTG | CGGTGATAGCCGCAACAATT | Start: 44 |
| Tm | 58.99 | 59.267 | End: 219 |
| Size | 22 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | CCGCTCAAATTAAGAGGCTTGG | AGTTCTTACCCGGTGATAGCC | Start: 45 |
| Tm | 59.902 | 59.239 | End: 229 |
| Size | 22 | 21 | 185 |
| Primer 5 details |
|---|
| Sequence | CGCTCAAATTAAGAGGCTTGGTT | CGGTGATAGCCGCAACAATT | Start: 46 |
| Tm | 59.809 | 59.267 | End: 219 |
| Size | 23 | 20 | 174 |
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