ssr_primer
Primer Details for TRINITY_DN65860_c10_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTGGGGAGGGCTTTGAAG | GGAGGTGGAAACAGACGAGG | Start: 78 |
| Tm | 59.887 | 60.037 | End: 199 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
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| Sequence | ACTTGGGGAGGGCTTTGAAG | TACCGTGCTGAGAGGACTGA | Start: 78 |
| Tm | 59.887 | 59.962 | End: 219 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
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| Sequence | ACTTGGGGAGGGCTTTGAAG | TGAGAGGACTGAGGAGGTGG | Start: 78 |
| Tm | 59.887 | 59.96 | End: 211 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
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| Sequence | CACTTGGGGAGGGCTTTGAA | GGAGGTGGAAACAGACGAGG | Start: 77 |
| Tm | 60.179 | 60.037 | End: 199 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | CACTTGGGGAGGGCTTTGAA | TACCGTGCTGAGAGGACTGA | Start: 77 |
| Tm | 60.179 | 59.962 | End: 219 |
| Size | 20 | 20 | 143 |
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