ssr_primer
Primer Details for TRINITY_DN65853_c8_g1_i1 |
|---|
| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGAGAATTCAAACGCCGA | AGCGGACAATATGATGCCCT | Start: 125 |
| Tm | 59.676 | 59.233 | End: 248 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AGGGAGAATTCAAACGCCGA | AGCGGACAATATGATGCCCTT | Start: 125 |
| Tm | 59.676 | 59.856 | End: 248 |
| Size | 20 | 21 | 124 |
| Primer 3 details |
|---|
| Sequence | AGGGAGAATTCAAACGCCGAT | AGCGGACAATATGATGCCCT | Start: 125 |
| Tm | 60.065 | 59.233 | End: 248 |
| Size | 21 | 20 | 124 |
| Primer 4 details |
|---|
| Sequence | AAGGGAGAATTCAAACGCCG | AGCGGACAATATGATGCCCT | Start: 124 |
| Tm | 58.835 | 59.233 | End: 248 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | AGGGAGAATTCAAACGCCGA | GCGGACAATATGATGCCCTT | Start: 125 |
| Tm | 59.676 | 58.391 | End: 247 |
| Size | 20 | 20 | 123 |
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