ssr_primer
Primer Details for TRINITY_DN65845_c1_g1_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCCCGAACTTGCCATG | AATCCGGCAACAGAGGACAG | Start: 382 |
| Tm | 60.107 | 60.036 | End: 507 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | GGATCCCGAACTTGCCATGA | AATCCGGCAACAGAGGACAG | Start: 383 |
| Tm | 60.107 | 60.036 | End: 507 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CTGTCCTCTGTTGCCGGATT | CGTGAGCCGATTTCAGTTGC | Start: 488 |
| Tm | 60.036 | 60.179 | End: 671 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | AGAAATTTTGGCGCGCCTAC | CATGGCAAGTTCGGGATCCT | Start: 221 |
| Tm | 59.83 | 60.107 | End: 401 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | AGAAATTTTGGCGCGCCTAC | TCATGGCAAGTTCGGGATCC | Start: 221 |
| Tm | 59.83 | 60.107 | End: 402 |
| Size | 20 | 20 | 182 |
(G)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCCCGAACTTGCCATG | AATCCGGCAACAGAGGACAG | Start: 382 |
| Tm | 60.107 | 60.036 | End: 507 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | GGATCCCGAACTTGCCATGA | AATCCGGCAACAGAGGACAG | Start: 383 |
| Tm | 60.107 | 60.036 | End: 507 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | CTGTCCTCTGTTGCCGGATT | CGTGAGCCGATTTCAGTTGC | Start: 488 |
| Tm | 60.036 | 60.179 | End: 671 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | AGAAATTTTGGCGCGCCTAC | CATGGCAAGTTCGGGATCCT | Start: 221 |
| Tm | 59.83 | 60.107 | End: 401 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | AGAAATTTTGGCGCGCCTAC | TCATGGCAAGTTCGGGATCC | Start: 221 |
| Tm | 59.83 | 60.107 | End: 402 |
| Size | 20 | 20 | 182 |
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