ssr_primer
Primer Details for TRINITY_DN65793_c4_g3_i1 |
|---|
| (A)9(ACAAACAAACAAACAAACAAACAAACAAAGCTGAAAACTGATCAATGTTATTTTTTAGAACCAATCAAACGATTATTTATTGCCACCTTGCTCCACCTTGTCTCTCAGCTTGAATTGTCGAAAAAAAGGCGAAGTAAAGCAGCCCTGCAAGTCTCCCTCTCCTCCCTGAAATGCTGCTTAATACAGGGTCGGACTTTCTCCCCCCTATATTTTATGAACAACCTCAAAGGTCCATTGCAACAAAAAGGGTTACGAGCATCATCGCTAGTTCGCTTGGCATCTTAGGATTCAGCTTGGAGATCCGGAAAAACAATGGCTTCTAAGGGTTCCGCTTGGAGATCCGGAAAACAAGAAGCTTTTCAGATTCTGCATAACGTTAGAGTGTGACAAGGAAGATATTCCGCGATGCGACAACAGCGACAACGAACGTTGATCGCGTTCGTGAAGAAGTCATGCGTAACAACCTGACTCCTGCTTCGTCTCTATGTGATCGTCTGATGCTCCGTTCATCAAAACCATCACCCCCTTCCCCCCACCACTGTATCATCGAAAATTGGCCAATGACCTACCGCGCCGATCTCGCGCACATGGCGAGTCGTTTACCCACCGCGTGGAGACCTAATTCTTCTTCTCATAAACGGATGGTCGAGTACCGATCCTGATGGCAGATCATGACCGAGGGTGAGAGGCCGCCCC)(AAAC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCTAAGGGTTCCGCTT | AACGTTCGTTGTCGCTGTTG | Start: 881 |
| Tm | 60.035 | 59.974 | End: 997 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | GGCTTCTAAGGGTTCCGCTT | CAACGTTCGTTGTCGCTGTT | Start: 881 |
| Tm | 60.035 | 59.974 | End: 998 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | CAACAGCGACAACGAACGTT | GCCATCAGGATCGGTACTCG | Start: 978 |
| Tm | 59.974 | 60.039 | End: 1232 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | GGCTTCTAAGGGTTCCGCTT | TGACTTCTTCACGAACGCGA | Start: 881 |
| Tm | 60.035 | 59.97 | End: 1019 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TCGCGTTCGTGAAGAAGTCA | GCCATCAGGATCGGTACTCG | Start: 1000 |
| Tm | 59.97 | 60.039 | End: 1232 |
| Size | 20 | 20 | 233 |
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