ssr_primer
Primer Details for TRINITY_DN65773_c1_g1_i3 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTTTCGCCTTTGATCGT | TGTTTACGAGCATGGCCCAT | Start: 11 |
| Tm | 59.964 | 60.035 | End: 210 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
|---|
| Sequence | ACCCTTTCGCCTTTGATCGT | ATGTTTACGAGCATGGCCCA | Start: 11 |
| Tm | 59.964 | 60.035 | End: 211 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | GCAATTAACGGGCAGTGAGC | TGTTTACGAGCATGGCCCAT | Start: 60 |
| Tm | 60.179 | 60.035 | End: 210 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | GCAATTAACGGGCAGTGAGC | ATGTTTACGAGCATGGCCCA | Start: 60 |
| Tm | 60.179 | 60.035 | End: 211 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | ACCCTTTCGCCTTTGATCGT | TGAGCAGTAATAGCCCGTGC | Start: 11 |
| Tm | 59.964 | 60.179 | End: 143 |
| Size | 20 | 20 | 133 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTTTCGCCTTTGATCGT | TGTTTACGAGCATGGCCCAT | Start: 11 |
| Tm | 59.964 | 60.035 | End: 210 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
|---|
| Sequence | ACCCTTTCGCCTTTGATCGT | ATGTTTACGAGCATGGCCCA | Start: 11 |
| Tm | 59.964 | 60.035 | End: 211 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | GCAATTAACGGGCAGTGAGC | TGTTTACGAGCATGGCCCAT | Start: 60 |
| Tm | 60.179 | 60.035 | End: 210 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | GCAATTAACGGGCAGTGAGC | ATGTTTACGAGCATGGCCCA | Start: 60 |
| Tm | 60.179 | 60.035 | End: 211 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | ACCCTTTCGCCTTTGATCGT | TGAGCAGTAATAGCCCGTGC | Start: 11 |
| Tm | 59.964 | 60.179 | End: 143 |
| Size | 20 | 20 | 133 |
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