Primer Details for TRINITY_DN65750_c1_g1_i1 |
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| (TTTA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGCAGTCAAATGAGGGGC | ATGCCTTAAGACTCGTTTTACTTGA | Start: 137 |
| Tm | 59.541 | 58.772 | End: 236 |
| Size | 20 | 25 | 100 |
| Primer 2 details |
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| Sequence | CATGCAGTCAAATGAGGGGC | ATGCCTTAAGACTCGTTTTACTTG | Start: 137 |
| Tm | 59.541 | 57.518 | End: 236 |
| Size | 20 | 24 | 100 |
| Primer 3 details |
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| Sequence | CATGCAGTCAAATGAGGGGC | ATGCCTTAAGACTCGTTTTACTTGAA | Start: 137 |
| Tm | 59.541 | 59.291 | End: 236 |
| Size | 20 | 26 | 100 |
| Primer 4 details |
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| Sequence | TCATGCAGTCAAATGAGGGGC | TGCCTTAAGACTCGTTTTACTTGA | Start: 136 |
| Tm | 60.957 | 58.396 | End: 235 |
| Size | 21 | 24 | 100 |
| Primer 5 details |
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| Sequence | CATGCAGTCAAATGAGGGGC | ATGCCTTAAGACTCGTTTTACTTGAAA | Start: 137 |
| Tm | 59.541 | 59.772 | End: 236 |
| Size | 20 | 27 | 100 |
