ssr_primer
Primer Details for TRINITY_DN65735_c1_g1_i4 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCGCAGTGATTCTCCAAT | GGGCATCACATCTTCCCCAA | Start: 424 |
| Tm | 60.035 | 60.033 | End: 699 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | ATTGGCGCAGTGATTCTCCA | GGGCATCACATCTTCCCCAA | Start: 422 |
| Tm | 60.035 | 60.033 | End: 699 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | GGACGGACGAGGTACAATCC | GGGCATCACATCTTCCCCAA | Start: 482 |
| Tm | 59.898 | 60.033 | End: 699 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
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| Sequence | TGGCGCAGTGATTCTCCAAT | AGCTGGGCATCACATCTTCC | Start: 424 |
| Tm | 60.035 | 60.107 | End: 703 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
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| Sequence | GGACGGACGAGGTACAATCC | AGCTGGGCATCACATCTTCC | Start: 482 |
| Tm | 59.898 | 60.107 | End: 703 |
| Size | 20 | 20 | 222 |
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