ssr_primer
Primer Details for TRINITY_DN65722_c6_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTCAAGGTGACTCCACGT | AACTTCTGGACGCCGGAAAT | Start: 215 |
| Tm | 59.894 | 59.964 | End: 419 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
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| Sequence | CCCCCGAAGTGCTCATCATT | AACTTCTGGACGCCGGAAAT | Start: 253 |
| Tm | 60.107 | 59.964 | End: 419 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | GGTGACTCCACGTCTCGAAG | AACTTCTGGACGCCGGAAAT | Start: 222 |
| Tm | 60.11 | 59.964 | End: 419 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
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| Sequence | CAAGTGCAACGCCGATGAAT | AACTTCTGGACGCCGGAAAT | Start: 274 |
| Tm | 59.831 | 59.964 | End: 419 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | GGTGGGGTTAGGAACTTGCA | ACGTGGAGTCACCTTGAACC | Start: 15 |
| Tm | 59.888 | 59.894 | End: 234 |
| Size | 20 | 20 | 220 |
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