ssr_primer
Primer Details for TRINITY_DN65712_c5_g1_i1 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACGTCTTGGGGGTAAGC | CCGTGATTCACCTGTTCCGA | Start: 296 |
| Tm | 59.964 | 60.038 | End: 460 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TCGCTCATGGTGACCTTGAC | CCGTGATTCACCTGTTCCGA | Start: 281 |
| Tm | 60.037 | 60.038 | End: 460 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | GTTGCGGAATTGACTCGAGC | ACTAAATATCCTGCGCGGGG | Start: 562 |
| Tm | 59.905 | 59.965 | End: 795 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TCGGAACAGGTGAATCACGG | GCTCGAGTCAATTCCGCAAC | Start: 441 |
| Tm | 60.038 | 59.905 | End: 581 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | TTGACGTCTTGGGGGTAAGC | CACCTGTTCCGAGATCCGAG | Start: 296 |
| Tm | 59.964 | 59.899 | End: 452 |
| Size | 20 | 20 | 157 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACGTCTTGGGGGTAAGC | CCGTGATTCACCTGTTCCGA | Start: 296 |
| Tm | 59.964 | 60.038 | End: 460 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TCGCTCATGGTGACCTTGAC | CCGTGATTCACCTGTTCCGA | Start: 281 |
| Tm | 60.037 | 60.038 | End: 460 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | GTTGCGGAATTGACTCGAGC | ACTAAATATCCTGCGCGGGG | Start: 562 |
| Tm | 59.905 | 59.965 | End: 795 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TCGGAACAGGTGAATCACGG | GCTCGAGTCAATTCCGCAAC | Start: 441 |
| Tm | 60.038 | 59.905 | End: 581 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | TTGACGTCTTGGGGGTAAGC | CACCTGTTCCGAGATCCGAG | Start: 296 |
| Tm | 59.964 | 59.899 | End: 452 |
| Size | 20 | 20 | 157 |
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