ssr_primer
Primer Details for TRINITY_DN65685_c3_g1_i5 |
|---|
| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACATTGGCCAGTTCTGCA | TTGCAACTGCCCTGAGAGAC | Start: 346 |
| Tm | 60.179 | 60.25 | End: 510 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | TGCAAGTGCGGGTAGAATCT | Start: 30 |
| Tm | 59.968 | 59.387 | End: 260 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | AGATGCAAGTGCGGGTAGAA | Start: 30 |
| Tm | 59.968 | 59.387 | End: 263 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | TGCAAGTGCGGGTAGAATCT | Start: 29 |
| Tm | 59.967 | 59.387 | End: 260 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | AGATGCAAGTGCGGGTAGAA | Start: 29 |
| Tm | 59.967 | 59.387 | End: 263 |
| Size | 20 | 20 | 235 |
(G)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACATTGGCCAGTTCTGCA | TTGCAACTGCCCTGAGAGAC | Start: 346 |
| Tm | 60.179 | 60.25 | End: 510 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | TGCAAGTGCGGGTAGAATCT | Start: 30 |
| Tm | 59.968 | 59.387 | End: 260 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TGGAGTTCAGACGTGTGCTC | AGATGCAAGTGCGGGTAGAA | Start: 30 |
| Tm | 59.968 | 59.387 | End: 263 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | TGCAAGTGCGGGTAGAATCT | Start: 29 |
| Tm | 59.967 | 59.387 | End: 260 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | AGATGCAAGTGCGGGTAGAA | Start: 29 |
| Tm | 59.967 | 59.387 | End: 263 |
| Size | 20 | 20 | 235 |
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