ssr_primer
Primer Details for TRINITY_DN65645_c6_g1_i3 |
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| (A)14caaacaaacagac(A)21(ACAA)5*: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCGGAGGGAATTGGACTC | TTTTCCGCCAAATGCCAAGG | Start: 231 |
| Tm | 60.035 | 59.965 | End: 354 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
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| Sequence | TCTTCTTGGACGTTTGCGGA | TTTTCCGCCAAATGCCAAGG | Start: 218 |
| Tm | 59.895 | 59.965 | End: 354 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | GGAGGGAATTGGACTCCGTG | TTTTCCGCCAAATGCCAAGG | Start: 235 |
| Tm | 60.108 | 59.965 | End: 354 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
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| Sequence | TTGCGGAGGGAATTGGACTC | GCAGAAGCCCGTTACCAGAT | Start: 231 |
| Tm | 60.035 | 60.108 | End: 332 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | GACGTTTGCGGAGGGAATTG | TTTTCCGCCAAATGCCAAGG | Start: 226 |
| Tm | 59.832 | 59.965 | End: 354 |
| Size | 20 | 20 | 129 |
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