ssr_primer
Primer Details for TRINITY_DN65644_c3_g1_i10 |
|---|
| (A)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCACGTTCTTGTCGGGAT | GTGACCGGGTGCTAAGTTCA | Start: 1428 |
| Tm | 60.038 | 59.966 | End: 1700 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | AGGGACGTACCTCATCGACA | ATCCCGACAAGAACGTGGAC | Start: 1268 |
| Tm | 60.035 | 60.038 | End: 1447 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | ATCAAGGGACACGAGCGTTT | ATCCCGACAAGAACGTGGAC | Start: 1307 |
| Tm | 59.965 | 60.038 | End: 1447 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | GTCCACGTTCTTGTCGGGAT | CCGGGTGCTAAGTTCAAGGT | Start: 1428 |
| Tm | 60.038 | 59.964 | End: 1696 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | GTCCACGTTCTTGTCGGGAT | ACCGGGTGCTAAGTTCAAGG | Start: 1428 |
| Tm | 60.038 | 59.964 | End: 1697 |
| Size | 20 | 20 | 270 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCACGTTCTTGTCGGGAT | GTGACCGGGTGCTAAGTTCA | Start: 1428 |
| Tm | 60.038 | 59.966 | End: 1700 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | AGGGACGTACCTCATCGACA | ATCCCGACAAGAACGTGGAC | Start: 1268 |
| Tm | 60.035 | 60.038 | End: 1447 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | ATCAAGGGACACGAGCGTTT | ATCCCGACAAGAACGTGGAC | Start: 1307 |
| Tm | 59.965 | 60.038 | End: 1447 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | GTCCACGTTCTTGTCGGGAT | CCGGGTGCTAAGTTCAAGGT | Start: 1428 |
| Tm | 60.038 | 59.964 | End: 1696 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | GTCCACGTTCTTGTCGGGAT | ACCGGGTGCTAAGTTCAAGG | Start: 1428 |
| Tm | 60.038 | 59.964 | End: 1697 |
| Size | 20 | 20 | 270 |
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