ssr_primer
Primer Details for TRINITY_DN65628_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACAATGTGCTTGGCTGAC | AAAAAGGGGAGGGGAGGAGG | Start: 2 |
| Tm | 60.039 | 60.851 | End: 132 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
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| Sequence | ATGTGCTTGGCTGACTGTGA | AAAAAGGGGAGGGGAGGAGG | Start: 7 |
| Tm | 59.891 | 60.851 | End: 132 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
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| Sequence | TCCACAATGTGCTTGGCTGA | AAAAAGGGGAGGGGAGGAGG | Start: 1 |
| Tm | 60.179 | 60.851 | End: 132 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | CTCCACAATGTGCTTGGCTG | AAAAAGGGGAGGGGAGGAGG | Start: 0 |
| Tm | 59.757 | 60.851 | End: 132 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | CCACAATGTGCTTGGCTGAC | AGTAAAAAGGGGAGGGGAGGA | Start: 2 |
| Tm | 60.039 | 59.844 | End: 135 |
| Size | 20 | 21 | 134 |
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