ssr_primer
Primer Details for TRINITY_DN65622_c1_g1_i1 |
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| (C)22gctcttacaaccttc(T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGAGGGTGCGTTCATTCT | ATTGGTGGGGTTGACTCAGG | Start: 147 |
| Tm | 60.036 | 59.594 | End: 285 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | CTGGAGGGTGCGTTCATTCT | GGTTGACTCAGGTGGCACAA | Start: 147 |
| Tm | 60.036 | 60.465 | End: 277 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | AGGGTGCGTTCATTCTGTGT | ATTGGTGGGGTTGACTCAGG | Start: 151 |
| Tm | 59.891 | 59.594 | End: 285 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | ACCCCCTCAAAAGGAGTGTG | AGAATGAACGCACCCTCCAG | Start: 65 |
| Tm | 59.521 | 60.036 | End: 166 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | CACCCCCTCAAAAGGAGTGT | AGAATGAACGCACCCTCCAG | Start: 64 |
| Tm | 59.521 | 60.036 | End: 166 |
| Size | 20 | 20 | 103 |
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