ssr_primer
Primer Details for TRINITY_DN65594_c4_g1_i1 |
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| (A)10ttctttggatacaaatccccaaaagggatcacgatgatttatcaaaac(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTAAGCCTCTGCCTACCT | GCCCGATTCCGAGGAAGAAT | Start: 143 |
| Tm | 60.324 | 59.893 | End: 386 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
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| Sequence | ACGTAAGCCTCTGCCTACCT | CTCGTTTCGAAAAGTGCCCG | Start: 143 |
| Tm | 60.324 | 60.111 | End: 401 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
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| Sequence | ACAGCGCGAGGTTGATTCTT | GGGGCTCACCTGTTACGTAG | Start: 353 |
| Tm | 60.321 | 59.825 | End: 460 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | CAGCGCGAGGTTGATTCTTC | GGGGCTCACCTGTTACGTAG | Start: 354 |
| Tm | 59.628 | 59.825 | End: 460 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
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| Sequence | AACGTAAGCCTCTGCCTACC | GCCCGATTCCGAGGAAGAAT | Start: 142 |
| Tm | 59.464 | 59.893 | End: 386 |
| Size | 20 | 20 | 245 |
(C)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTAAGCCTCTGCCTACCT | GCCCGATTCCGAGGAAGAAT | Start: 143 |
| Tm | 60.324 | 59.893 | End: 386 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
|---|
| Sequence | ACGTAAGCCTCTGCCTACCT | CTCGTTTCGAAAAGTGCCCG | Start: 143 |
| Tm | 60.324 | 60.111 | End: 401 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | ACAGCGCGAGGTTGATTCTT | GGGGCTCACCTGTTACGTAG | Start: 353 |
| Tm | 60.321 | 59.825 | End: 460 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | CAGCGCGAGGTTGATTCTTC | GGGGCTCACCTGTTACGTAG | Start: 354 |
| Tm | 59.628 | 59.825 | End: 460 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | AACGTAAGCCTCTGCCTACC | GCCCGATTCCGAGGAAGAAT | Start: 142 |
| Tm | 59.464 | 59.893 | End: 386 |
| Size | 20 | 20 | 245 |
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