ssr_primer
Primer Details for TRINITY_DN65575_c4_g4_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATCCCGATTGTGCAGTCG | AGCTGTCAGGGTGTCCACTA | Start: 143 |
| Tm | 60.179 | 60.179 | End: 378 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
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| Sequence | AGATCCCGATTGTGCAGTCG | TCCACTAAAACAGGCTGGCC | Start: 143 |
| Tm | 60.179 | 60.251 | End: 365 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
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| Sequence | TTGCCCGAAAGATCCCGATT | AGCTGTCAGGGTGTCCACTA | Start: 134 |
| Tm | 59.745 | 60.179 | End: 378 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
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| Sequence | TTTGCCCGAAAGATCCCGAT | AGCTGTCAGGGTGTCCACTA | Start: 133 |
| Tm | 59.745 | 60.179 | End: 378 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | ATTTGCCCGAAAGATCCCGA | AGCTGTCAGGGTGTCCACTA | Start: 132 |
| Tm | 59.745 | 60.179 | End: 378 |
| Size | 20 | 20 | 247 |
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